Well, his worst work is Eine Kleine Nacht Musik. It is musical popcorn.
But the guy wrote 600-odd pieces of music, so he is allowed the odd bad one.
The most wierd one he composed was for glass harmonium. This was a mechanically driven set of rotating glasses tuned to different notes, and sounded by a keyboard linked to felt pads which pressed on the rim. Kind of like the thing we've all done of rubbing a finger around a part full wine glass. The guy who funded that piece clearly had bought this instrument from hell, and Mozart was commissioned to write something for it.
But I have no idea how you would write a list of his best pieces. The operas? The clarinet concerto and clarinet quintet? The requiem? I love everthing I've heard of his, with the exception of EKNM, which drives my nuts.